Anti-HOXA9 Rabbit Polyclonal Antibody for WB and ELISA - P31269

Core Product Specifications and Parameters

Biological Background: HOXA9 Function and Localization

• HOXA9 is a homeobox transcription factor that provides cells with specific positional identities along the anterior‑posterior axis during development; it is also known as homeobox protein Hox‑1G.
• Classified as a proto‑oncogene, HOXA9 is involved in chromosomal rearrangements frequently observed in acute leukemias, underscoring its role in hematopoietic regulation.
• It binds DNA in a sequence‑specific manner to control the expression of genes essential for embryogenesis and cell fate determination, and it is a key member of the HOX gene family.
• HOXA9 is required for the upregulation of E‑selectin (SELE) and VCAM1 on the endothelial cell surface at sites of inflammation, facilitating leukocyte adhesion and extravasation. Related references: PMID:22269951
• In the cytoplasm, HOXA9 enhances EIF4E‑mediated mRNA nuclear export and boosts the translation efficiency of ODC mRNA by competing with repressive factors such as PRH, linking it to post‑transcriptional control.
• Although primarily nuclear, HOXA9 also localizes to the cytoplasm, and its activity is regulated by methylation and other post‑translational modifications.
• UniProt keywords highlight its roles as a developmental protein, DNA‑binding factor, and transcription regulator, as well as its involvement in chromosomal rearrangement and its proto‑oncogenic potential.

Experimental Guidance and Technical Tips

• A band at approximately 30 kDa is expected in western blotting; consider using nuclear‑enriched lysates, as HOXA9 functions predominantly in the nucleus.
• The immunogen covers amino acids 1–190, which includes the N‑terminal region outside the homeodomain; polyclonal reactivity may detect post‑translationally modified forms or splice variants—validate in your relevant sample system.
• For ELISA, perform a matrix check with known positive and negative controls (e.g., recombinant protein vs. pre‑immune serum) to establish appropriate cut‑off values.
• Cross‑reactivity with mouse is indicated; always confirm performance in mouse tissues or cell lines by titration and comparison with human controls.
• Storage and handling as per the ice‑bag shipping condition: aliquot upon arrival and avoid repeated freeze‑thaw cycles to maintain activity.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, assay developers, and research laboratories with a streamlined path to high‑quality IVD raw materials. This anti‑HOXA9 polyclonal antibody is ideally suited for partners focusing on developmental biology, hematological malignancy assessment, and inflammation‑related biomarker detection. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary proteins, and customized rare‑target sourcing—all designed to accelerate your development workflow from concept to clinical application.