Anti-HOXA9 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P31269

Core Product Specifications and Parameters

Biological Background: HOXA9 Function and Localization

• HOXA9 is a homeobox-containing transcription factor (alternative names: HOX1G, ABD-B-like) that provides cells with specific positional identities along the anterior-posterior axis during development.
• It acts as a sequence-specific DNA-binding protein that regulates gene expression programs critical for embryonic patterning and hematopoiesis.
• In endothelial cells, HOXA9 is required for the induction of SELE (E-selectin) and VCAM1 at sites of inflammation. Related references: PMID:22269951
• HOXA9 positively regulates EIF4E-mediated mRNA nuclear export and enhances the translation efficiency of ODC mRNA by competing with EIF4E repressors such as PRH (by similarity).
• The protein localizes to both the nucleus and cytoplasm, consistent with its dual roles in transcriptional regulation and post-transcriptional control.
• HOXA9 contains a characteristic homeobox DNA-binding domain and is subject to methylation and chromosomal rearrangements; it is implicated as a proto-oncogene in certain leukemias.

Experimental Guidance and Technical Tips

• For Western blot, a band of approximately 30 kDa is expected; validate band specificity using positive and negative cell lysates.
• In immunohistochemistry (IHC-P), anticipate nuclear and/or cytoplasmic staining patterns; include appropriate tissue controls and optimize antigen retrieval conditions.
• When designing ELISA assays, consider pairing with a capture antibody targeting a different epitope; cross-reactivity with rat HOXA9 should be confirmed in relevant sample matrices.
• As the immunogen spans amino acids 173–272, the antibody may detect isoforms or fragments containing this region; confirm performance in the intended application with endogenous controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a seamless procurement experience for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets. This HOXA9 antibody supports research in developmental biology, oncology, and transcription regulation, and our team can assist with scaling supply or custom development needs to move your project from concept to clinic.