Anti-HOXA13 Rabbit Polyclonal Antibody for WB and ELISA - P31271

Core Product Specifications and Parameters

Biological Background: HOXA13 Function and Localization

• HOXA13 (Homeobox protein Hox-A13), also known as HOX1J, is a member of the homeobox family of transcription factors characterized by a conserved DNA-binding homeodomain.
• It functions as a sequence-specific, AT-rich binding transcription factor that provides cells with specific positional identities along the anterior-posterior axis during development.
• HOXA13 is part of the HOXA gene cluster on chromosome 7 and plays a critical role in limb, genitourinary, and gastrointestinal tract morphogenesis.
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation of target genes involved in developmental processes.
• Mutations in HOXA13 are associated with human disease variants, including hand-foot-genital syndrome and Guttmacher syndrome, highlighting its developmental significance.
• HOXA13 is classified under keywords including developmental protein, DNA-binding, homeobox, and transcription regulation, with its structure characterized by a flexible N-terminal region and a conserved homeodomain.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 140–320 of human HOXA13 (NP_000513.2), covering the region adjacent to the homeodomain; consider that the antibody may detect full-length HOXA13 and potentially isoforms containing this sequence.
• For Western blotting, a band around 40 kDa is expected; optimize loading and transfer conditions given the moderate molecular weight.
• When using ELISA, validate the antibody’s performance with your specific coating and detection protocols to ensure linearity and sensitivity.
• Cross-reactivity with mouse and rat HOXA13 is reported; however, sequence homology should be assessed for your specific experimental model to minimize off-target effects.
• As a rabbit polyclonal antibody, it may exhibit lot-to-lot variability; it is advisable to titrate and validate each new lot in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, supplying this HOXA13 polyclonal antibody alongside a broad portfolio of antibodies and reagents. From early development to clinical manufacturing, we provide reliable access to rare target materials and customized supply solutions to accelerate your project.