Anti-HOXA13 Polyclonal Antibody for WB, ELISA - P31271

Core Product Specifications and Parameters

Biological Background: HOXA13 Function and Localization

• Homeobox protein Hox-A13 (also known as Hox-1J) is a member of the HOX family of transcription factors critical for anterior-posterior body axis patterning during embryonic development.
• Functions as a sequence-specific, AT-rich DNA-binding transcription factor that regulates target gene expression to confer positional identities to cells along the anteroposterior axis.
• Localizes predominantly to the nucleus, consistent with its role as a DNA-binding transcriptional regulator.
• Contains a conserved homeobox domain responsible for DNA binding and transcriptional regulation.
• Classified under developmental proteins and DNA-binding proteins; implicated in developmental disorders as indicated by disease variant annotations.
• The calculated molecular weight of human HOXA13 is approximately 39.7 kDa (based on UniProt; observed product band at ~40 kDa in applications).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 140-320 of human HOXA13, covering a region that includes part of the homeodomain and adjacent sequences. Validate antibody specificity in your system, as polyclonal antibodies may cross-react with other HOX family members.
• For Western blot (WB), consider using nuclear extracts or lysates from human cell lines known to express HOXA13. A band near 40 kDa is expected; verify with appropriate positive and negative controls.
• In ELISA applications, this antibody can be used as a detection or capture antibody. Optimize coating conditions and antibody concentrations for your specific assay format.
• Always perform a preliminary titration experiment to determine the optimal working dilution for your specific application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-HOXA13 polyclonal antibody supports research into developmental biology and transcriptional regulation, and CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to meet your specific diagnostic and research needs.