Anti-HOXA13 Monoclonal Antibody for WB, ELISA - P31271

Core Product Specifications and Parameters

Biological Background: HOXA13 Function and Localization

• HOXA13 is a homeobox-containing transcription factor that binds DNA in a sequence-specific manner, preferentially recognizing AT-rich sequences, to regulate gene expression.
• It is a member of the HOXA gene cluster on chromosome 7, playing a critical role in embryonic development by providing cells with positional identities along the anterior-posterior body axis.
• HOXA13 functions as a developmental regulatory protein, essential for proper morphogenesis of the distal limbs and genitourinary tract.
• The protein localizes to the nucleus, consistent with its role as a transcription factor.
• Post-translational modifications and disease-associated variants of HOXA13 have been linked to hand-foot-genital syndrome and other congenital anomalies.
• Its expression is tightly regulated during embryogenesis, with continued expression in adult tissues where it may contribute to tissue homeostasis.

Experimental Guidance and Technical Tips

• The immunogen sequence corresponds to a region between amino acids 250 and 350 of human HOXA13, which is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, consider using nuclear extracts or whole-cell lysates from developmental tissues or cell lines expressing HOXA13; a band around 40 kDa is expected.
• In ELISA, validate the antibody's specificity using recombinant HOXA13 protein or peptide competition assays.
• Due to homeodomain conservation, verify that the antibody does not cross-react with other HOX family members if specificity is critical for your application; epitope mapping may help.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, supplying validated antibodies like this anti-HOXA13 monoclonal antibody for developmental biology and transcription research. We offer bulk quantities, optimized antibody pairs, and customized sourcing for rare targets to streamline your diagnostic assay development.