Anti-HOXA11 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P31270

Core Product Specifications and Parameters

Biological Background: HOXA11 Function and Localization

• HOXA11 (homeobox protein Hox-A11) is a member of the HOX family of transcription factors, encoded by the HOXA11 gene (also designated HOX1I).
• It binds DNA in a sequence-specific manner through its conserved homeodomain and regulates the expression of target genes.
• As part of the HOX gene cluster, it provides cells with positional identity along the anterior-posterior axis during embryonic development.
• The protein is localized exclusively to the nucleus, consistent with its function as a transcriptional regulator.
• HOXA11 is classified as a developmental protein and contains a homeobox DNA-binding domain characteristic of the Antennapedia-type homeobox family.
• It is included in the human reference proteome, underscoring its fundamental role in biology.

Experimental Guidance and Technical Tips

• The immunogen used spans the N‑terminal region (amino acids 1–180), which may exhibit lower conservation among HOX family members; cross‑reactivity with other HOX proteins is possible but might be reduced compared to homeodomain‑targeting antibodies.
• For Western blotting, a band around 34 kDa is expected for HOXA11; always include appropriate controls (e.g., positive lysates from cell lines known to express HOXA11) to confirm specificity.
• In immunofluorescence/ICC experiments, ensure thorough permeabilization of cells to allow antibody access to the nuclear compartment.
• The antibody is suitable for ELISA; consider pairing it with another HOXA11‑specific antibody for sandwich assays if required, and validate performance in your specific matrix.
• Since cross‑reactivity with mouse and rat is indicated, this antibody can be used in rodent models; however, we recommend validating reactivity in your tissue or cell system of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic developers and researchers from concept to clinic. For studies focused on homeobox transcription factors and developmental biology, we supply this anti-HOXA11 polyclonal antibody alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Our integrated supply chain and technical expertise help streamline your assay development and production workflows.