Anti-HOXA10 Polyclonal Antibody for WB, ELISA - P31260

Core Product Specifications and Parameters

Biological Background: HOXA10 Function and Localization

• HOXA10 is a homeobox protein that functions as a sequence-specific transcription factor, providing cells with positional identities along the anterior-posterior axis during development.
• It belongs to the HOX family of genes, which are master regulators of embryonic development and body plan formation.
• HOXA10 binds to the specific DNA sequence 5'-AA[AT]TTTTATTAC-3', regulating the expression of downstream target genes.
• The protein localizes predominantly to the nucleus, consistent with its role in transcriptional regulation.
• Alternative splicing produces multiple transcript variants, contributing to functional diversity.
• As a developmental protein, HOXA10 is crucial for proper morphogenesis and cellular differentiation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 236-336, a region potentially less conserved; the antibody may recognize multiple isoforms due to its polyclonal nature.
• For Western blotting, a band around 42 kDa is expected; verify expression in positive controls such as HeLa or mouse embryo lysates.
• For ELISA, titrate the antibody to establish optimal working concentration; use recombinant HOXA10 protein as a standard.
• Consider cross-reactivity with mouse and rat orthologs; validate in the relevant species' samples before extensive use.
• As a rabbit polyclonal, this antibody is suitable for applications requiring high sensitivity and recognition of multiple epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This HOXA10 antibody is part of our extensive portfolio of transcription factor antibodies for developmental biology and gene regulation studies. We also offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your research and diagnostic development.