Anti-HOPX Polyclonal Antibody for WB, IF-P, ELISA - Q9BPY8

Core Product Specifications and Parameters

Biological Background: HOPX Function and Localization

• Encoded by the HOPX gene (aliases HOD, HOP, OB1, LAGY, TOTO, CAMEO, NECC1, SMAP31); produces an atypical homeodomain protein that does not bind DNA.
• Functions as a transcriptional repressor by interacting with serum response factor (SRF) to modulate SRF-dependent cardiac-specific gene expression; overexpression leads to cardiac hypertrophy.
• Acts as a tumor suppressor and is frequently down-regulated in cancers such as lung cancer, choriocarcinoma, and head and neck squamous cell carcinoma.
• Serves as a co-chaperone for HSPA1A and HSPA1B, assisting in chaperone-mediated protein refolding.
  Related references: PMID:27708256
• Localizes to both the nucleus and cytoplasm.
• Widely expressed in heart, brain, placenta, lung, skeletal and smooth muscle, uterus, urinary bladder, kidney, and spleen; expression is reduced in several tumor types.
• Classified under keywords: alternative splicing, developmental protein, homeobox, proteomics identification, proto-oncogene, repressor, transcription, and transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1–73, covering the full-length HOPX protein; the antibody is expected to detect both nuclear and cytoplasmic pools.
• For Western blot, HOPX is a small protein (~8 kDa); use a 15% or gradient gel and optimize transfer conditions for low-molecular-weight targets.
• In immunofluorescence/IF paraffin, both nuclear and cytoplasmic signals may be observed depending on cell type and context; include appropriate controls.
• Validation is recommended in tissue/cell systems known to express HOPX (e.g., cardiac muscle, lung, or cancer cell lines with differential HOPX levels).
• For ELISA applications, consider pairing with a validated capture/detection system if used in a quantitative assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research labs with one-stop access to validated IVD raw materials. This anti-HOPX antibody addresses targets in cardiac biology, oncology, and chaperone pathways. Beyond bulk polyclonal and monoclonal antibodies, CamelBio can source antibody pairs, ancillary reagents, and rare-target raw materials, providing a streamlined procurement experience from discovery to IVD assay development.