Anti-HOOK2 Rabbit Monoclonal Antibody for WB, ELISA - Q96ED9

Core Product Specifications and Parameters

Biological Background: HOOK2 Function and Localization

• Protein Hook homolog 2 (HOOK2) is encoded by the HOOK2 gene and undergoes alternative splicing, potentially producing multiple isoforms.
• HOOK2 is a component of the FTS/Hook/FHIP (FHF) complex, which is implicated in promoting vesicle trafficking and fusion, likely through interaction with the homotypic vesicular protein sorting (HOPS) complex.
• It contributes to centrosome function and is involved in the positioning or formation of aggresomes — pericentriolar accumulations of misfolded proteins, proteasomes, and chaperones.
• The FHF complex facilitates the distribution of the AP-4 complex to the perinuclear region of the cell. Related references: PMID:32073997
• Subcellularly, HOOK2 localizes to the cytoplasm, centrosome, microtubule organizing center, and the trans-Golgi network.
• The protein contains coiled-coil domains and is post-translationally modified by phosphorylation, characteristic of proteins involved in intracellular trafficking and cytoskeleton organization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human HOOK2; consider epitope mapping for precise binding characterization.
• This antibody is suitable for Western blot and ELISA; validate specificity in relevant cell or tissue lysates from human, mouse, or rat to confirm performance.
• For detection of endogenous HOOK2, test multiple sample types given its broad tissue expression and roles in vesicle trafficking and centrosome biology.
• Pair this HOOK2 monoclonal antibody with appropriate secondary reagents and consider including positive/negative controls to ensure reproducibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material procurement, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to accelerate your diagnostic development. For researchers focusing on centrosome biology and intracellular trafficking, we provide reliable raw materials including HOOK2 monoclonal antibodies to support your assay development from concept to clinic.