Anti-HOOK1 Rabbit Polyclonal Antibody for WB, ELISA - Q9UJC3

Core Product Specifications and Parameters

Biological Background: HOOK1 Function and Localization

• HOOK1 encodes Protein Hook homolog 1, a cytoplasmic protein that is a component of the FTS/Hook/FHIP (FHF) complex.
  Related references:
  PMID:18799622 PMID:32073997

• The FHF complex is implicated in promoting vesicle trafficking and fusion through interaction with the homotypic vesicular protein sorting (HOPS) complex.
  Related references:
  PMID:18799622

• The FHF complex facilitates the perinuclear distribution of the AP-4 complex, a component of the intracellular sorting machinery.
  Related references:
  PMID:32073997

• HOOK1 is essential for spermatid differentiation, likely by positioning microtubules of the manchette and flagellum relative to the membrane skeleton (by similarity).

• Subcellularly, HOOK1 localizes to the cytoplasm and associates with the cytoskeleton, particularly microtubules.

• The protein is subject to post-translational modifications including acetylation and phosphorylation, and exists in multiple isoforms through alternative splicing.

• HOOK1 is classified under keywords such as developmental protein, differentiation, spermatogenesis, and protein transport, reflecting its role in male germ cell development.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fragment (aa 489-728) of human HOOK1; consider validating antibody specificity in HOOK1-expressing cell lines or tissues such as testis.
• For Western blotting, a band around 85 kDa is expected; include appropriate positive and negative controls.
• The antibody shows cross-reactivity with mouse and rat HOOK1, making it suitable for rodent model studies; however, verify performance in your specific experimental system.
• For ELISA, optimize coating antigen concentration and antibody dilution according to your assay requirements.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop platform for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This HOOK1 antibody is relevant for studies in developmental biology, spermatogenesis, and intracellular trafficking. Beyond this polyclonal antibody, CamelBio provides validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare-target raw materials to streamline your assay development.