Anti-HOMER1 Rabbit Polyclonal Antibody (WB, IF/ICC, ELISA) - Q86YM7

Core Product Specifications and Parameters

Biological Background: HOMER1 Function and Localization

• HOMER1 (Homer protein homolog 1) is a postsynaptic density scaffolding protein encoded by the HOMER1 gene, existing as multiple isoforms (isoform 1 and isoform 3) with distinct roles in neuronal signaling.
• It binds and cross-links cytoplasmic regions of metabotropic glutamate receptors (GRM1, GRM5), inositol 1,4,5-trisphosphate receptor type 1 (ITPR1), and other synaptic proteins (DNM3, RYR1, RYR2, SHANK1, SHANK3), physically coupling surface receptors to intracellular calcium release from the endoplasmic reticulum.
• Isoform 1 regulates trafficking and surface expression of GRM5, while isoform 3 acts as a natural dominant negative, dynamically competing with isoform 1 to modulate metabotropic glutamate function and contribute to synaptic plasticity and development.
• HOMER1 forms high-order complexes with SHANK1, essential for the structural and functional integrity of dendritic spines, which are key sites of excitatory synaptic transmission.
• Beyond the nervous system, HOMER1 negatively regulates T cell activation by competing with calcineurin/PPP3CA for NFAT protein binding, thereby inhibiting the calcineurin-NFAT signaling pathway. Related references: PMID:18218901
• Subcellularly, HOMER1 localizes to the cytoplasm, postsynaptic density, synapse, and dendritic spines, with implications in synaptic architecture and signal transduction.
• Post-translational modifications include acetylation and phosphorylation, as indicated by proteomics data; the protein also features a coiled-coil domain critical for its multimerization and scaffolding functions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 71-170, a conserved region across human and mouse HOMER1; test cross-reactivity in mouse models as expected.
• For WB, detect a band at approximately 40 kDa; consider using appropriate positive control lysates from human or mouse brain or neuronal cell lines.
• In IF/ICC, HOMER1 exhibits punctate staining at postsynaptic sites; validate antibody specificity using neuronal cultures (e.g., primary hippocampal neurons) and confirm co-localization with synaptic markers.
• ELISA can be performed using the immunizing peptide or recombinant HOMER1 protein as coating antigen; optimize antibody dilution in the relevant sample matrix.

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