Anti-Homer1 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - Q86YM7

Core Product Specifications and Parameters

Biological Background: Homer1 Function and Localization

• Homer1 is a postsynaptic density scaffolding protein encoded by the HOMER1 gene. Multiple isoforms (e.g., isoforms 1 and 3) are produced by alternative splicing, with isoform 3 acting as a dominant negative regulator.
• It binds and cross-links group I metabotropic glutamate receptors (GRM1 and GRM5) with intracellular proteins such as ITPR1, SHANK1, and SHANK3, coupling surface receptor activation to intracellular calcium release from the endoplasmic reticulum.
• Isoform 1 regulates the trafficking and surface expression of GRM5, while isoform 3 dynamically competes with isoform 1 to fine-tune synaptic metabotropic glutamate function and participates in structural changes during long-lasting neuronal plasticity and development.
• Through its association with SHANK1, Homer1 forms a high-order complex critical for the structural and functional integrity of dendritic spines (By similarity).
• Beyond the nervous system, Homer1 negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway, competing with calcineurin for NFAT protein binding and preventing NFAT dephosphorylation and activation. Related references: PMID:18218901
• Subcellularly, Homer1 is predominantly localized to the cytoplasm, postsynaptic density, excitatory synapses, and dendritic spines of neurons.
• The protein is subject to post-translational modifications such as acetylation and phosphorylation, and contains a coiled-coil domain important for multimerization.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 170–354, a region that may include coiled-coil or linker sequences; the antibody may recognize all Homer1 isoforms sharing this epitope.
• For Western blot, use reducing conditions and a suitable positive control such as human or rat brain lysate; expected band size is approximately 40 kDa, though isoform-specific migration may vary.
• In immunofluorescence and immunohistochemistry, optimize fixation/permeabilization protocols and, for paraffin sections, determine the appropriate antigen retrieval conditions to preserve epitope accessibility.
• Consider verifying isoform specificity via siRNA knockdown or isoform-specific constructs if discrimination between Homer1A, Homer1B, and Homer1C is needed.
• Antibody performance in ELISA can be assessed using recombinant Homer1 protein or cell lysates, with attention to blocking and wash buffer composition to reduce nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, contract research organizations, and academic labs with streamlined access to IVD raw materials, technical services, and consulting—from concept to clinical application. This anti-Homer1 antibody strengthens our neuroscience-focused portfolio, which includes validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and custom sourcing for rare or emerging targets. Let CamelBio serve as your integrated procurement partner, helping you accelerate assay development with confidence.