Anti-Homer1 Rabbit Monoclonal Antibody for WB, ELISA - Q86YM7

Core Product Specifications and Parameters

Biological Background: Homer1 Function and Localization

• Homer1 is a postsynaptic density scaffolding protein that belongs to the Homer family and is encoded by the HOMER1 gene (alternative name SYN47). The protein exists in multiple isoforms produced by alternative splicing, including isoforms 1, 2, and 3, with distinct functional roles.
• It binds and cross-links group I metabotropic glutamate receptors (GRM1, GRM5) with endoplasmic reticulum-associated inositol 1,4,5-trisphosphate receptors (ITPR1), thereby coupling surface receptor activation to intracellular calcium release.
• Homer1 also links GRM1 to PI3 kinase through interaction with AGAP2, and forms high-order complexes with SHANK1, which is critical for dendritic spine structural and functional integrity.
• Isoform 1 regulates the trafficking and surface expression of GRM5, whereas isoform 3 acts as a natural dominant negative, competing with isoform 1 to modulate synaptic metabotropic glutamate function during long-lasting neuronal plasticity and development.
• Homer1 localizes to the cytoplasm, postsynaptic density, synapse, and dendritic spines, consistent with its role in synaptic organization.
• It negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway, competing with calcineurin for NFAT protein binding and preventing NFAT activation. Related references: PMID:18218901
• Post-translational modifications include acetylation and phosphorylation, as indicated by proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 101–220 of human Homer1, a central region that may include functional domains critical for antibody specificity. When designing similar antibodies, consider regions that are conserved across isoforms to achieve broad reactivity, or isoform-specific sequences if isoform-selective detection is required.
• For Western blot, the expected band is around 40 kDa; use fresh or properly frozen tissue/cell lysates, and consider including phosphatase inhibitors as Homer1 is a phosphoprotein.
• ELISA applications may be used to quantify Homer1 in samples; ensure proper coating conditions and validate linearity with recombinant protein standards.
• Given Homer1's synaptic localization, subcellular fractionation or enrichment of postsynaptic density proteins may enhance detection sensitivity in neuronal samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined one-stop sourcing solution for IVD raw materials, from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, supporting neuroscience and synaptic biology research. For rare or emerging targets like Homer1, CamelBio leverages extensive supply networks to deliver high-quality raw materials and technical support, accelerating your assay development.