Anti-HNRPLL Rabbit Polyclonal Antibody for WB, ELISA - Q8WVV9

Core Product Specifications and Parameters

Biological Background: HNRPLL Function and Localization

• Heterogeneous nuclear ribonucleoprotein L-like (HNRPLL), also known as stromal RNA-regulating factor (SRRF), is an RNA-binding protein encoded by the HNRNPLL gene in humans.
• It functions as a regulator of alternative splicing for multiple target mRNAs, including PTPRC/CD45 and STAT5A, critical for proper immune cell signaling and development.
• HNRPLL contains RNA recognition motifs and belongs to the heterogeneous nuclear ribonucleoprotein family, which is involved in pre-mRNA processing and nucleocytoplasmic transport.
• The protein is widely expressed across tissues, with detectable levels in bone marrow stroma cells, skeletal muscle, heart, placenta, pancreas, kidney, and lung.
• Post-translational modifications include phosphorylation and isopeptide bond formation (via ubiquitin conjugation), suggesting dynamic regulation of its activity and stability.
• Its molecular weight is approximately 60 kDa, consistent with the calculated mass of 60,083 Da, and it has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region spanning amino acids 250–350, which is less conserved and may contribute to the specificity of the antibody; however, cross-reactivity with similar proteins should be verified in the relevant sample system.
• For Western blotting, detect HNRPLL as a band near 60 kDa in whole cell lysates from human, mouse, or rat tissues (e.g., kidney, lung) and validate loading controls accordingly.
• For ELISA, consider coating with recombinant HNRPLL protein or the specific immunogen fragment; optimize antibody dilution and detection conditions based on the assay format.
• As a polyclonal antibody, lot-to-lot consistency should be confirmed for quantitative assays; include appropriate positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-HNRPLL antibody supports studies on alternative splicing and RNA biology, areas critical for understanding gene regulation and disease mechanisms. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your development workflows.