Anti-HNRPAB Polyclonal Antibody for WB, IF/ICC, ELISA - Q99729

Core Product Specifications and Parameters

Biological Background: HNRPAB Function and Localization

• HNRPAB (HNRNPAB) is a heterogeneous nuclear ribonucleoprotein (hnRNP) that binds single-stranded RNA with high affinity for G-rich and U-rich regions of heterogeneous nuclear RNA (hnRNA).
• Also known as APOBEC1-binding protein 1 (ABBP-1), it binds to APOB mRNA transcripts around the RNA editing site, suggesting a role in mRNA processing and editing.
• The protein is encoded by the HNRNPAB gene, which undergoes alternative splicing, potentially generating multiple isoforms.
• Subcellular localization is primarily nuclear, consistent with its role in pre-mRNA processing, but it is also found in the cytoplasm, possibly involved in mRNA transport or stability.
• Post-translational modifications include acetylation, phosphorylation, methylation, isopeptide bond formation, and ubiquitin-like conjugation, which may regulate its activity and interactions.
• Ubiquitously expressed across tissues, reflecting its fundamental role in RNA metabolism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–285, covering a large N-terminal region; cross-reactivity with splice variants or isoforms sharing this region may occur—validate specificity in your sample system.
• For Western blot (WB), a band around 36 kDa is expected; consider using appropriate molecular weight markers and loading controls.
• In immunofluorescence (IF/ICC), nuclear staining is anticipated; verify localization with a nuclear marker or DAPI co-stain.
• As a polyclonal antibody, lot-to-lot consistency should be monitored; re-titration and validation of new lots are recommended when transitioning.
• When using ELISA, optimize coating antigen and antibody concentrations; recombinant protein or peptide may be used for standard curves.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with access to high-quality IVD raw materials, including validated antibodies like this anti-HNRPAB polyclonal antibody for RNA biology and mRNA editing research. Our supply capabilities encompass bulk monoclonal and polyclonal antibodies, optimized antibody pairs, ancillary reagents, and sourcing of rare-target raw materials to support your assay development. Let CamelBio streamline your procurement from concept to clinic.