Anti-HNRNPM Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P52272

Core Product Specifications and Parameters

Biological Background: HNRNPM Function and Localization

• Heterogeneous nuclear ribonucleoprotein M (HNRNPM) is an RNA-binding protein encoded by the HNRNPM gene, also known as NAGR1, HTGR1, or HNRPM.
• HNRNPM functions as a pre-mRNA binding protein, exhibiting strong in vitro affinity for poly(G) and poly(U) RNA homopolymers, and plays a role in mRNA splicing.
• It acts as a receptor for carcinoembryonic antigen (CEA) on Kupffer cells, initiating signaling cascades that induce tyrosine phosphorylation and cytokine production, including IL-1 alpha, IL-6, IL-10, and tumor necrosis factor alpha.
• Subcellularly, HNRNPM localizes predominantly to the nucleus and nucleolus, consistent with its role in RNA processing.
• Post-translational modifications of HNRNPM include phosphorylation, methylation, acetylation, and ubiquitin-like conjugation, which may regulate its function and interactions.
• It is associated with the spliceosome complex and participates in alternative splicing, supported by its detection in multiple splice isoform variants.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 491-730) of human HNRNPM; consider that the antibody may detect multiple isoforms or variants arising from alternative splicing.
• In western blot (WB), a primary band near 78 kDa is anticipated, though additional bands may appear due to post-translational modifications or isoform diversity; validate in the relevant sample system.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), nuclear/nucleolar staining is expected; optimization of antigen retrieval and antibody dilution may be required for tissue-specific contexts.
• This polyclonal antibody is suitable for ELISA applications; ensure appropriate coating conditions and controls are used to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research institutions to streamline IVD raw-material procurement. For HNRNPM research in RNA splicing and cancer biology, we can supply optimized antibody pairs, bulk polyclonal and monoclonal antibodies, and ancillary reagents. Our team also supports rare target sourcing, helping you move from development to clinical application efficiently.