Anti-HNRNPL Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P14866

Core Product Specifications and Parameters

Biological Background: HNRNPL Function and Localization

• Heterogeneous nuclear ribonucleoprotein L (hnRNP-L) is an RNA-binding protein encoded by the HNRNPL gene; it belongs to the hnRNP family and participates in pre-mRNA processing and splicing regulation.
• Exhibits sequence-specific RNA binding, preferring CA-rich elements in exons or introns, acting as either a splicing activator or repressor of exon inclusion. Related references: PMID:11809897, PMID:22570490, PMID:24164894
• As a core component of heterogeneous nuclear ribonucleoprotein (hnRNP) complexes, it associates with most nascent transcripts and plays a key role in co-transcriptional RNA processing. Related references: PMID:2687284
• Contributes to transcriptional regulation; for example, it binds the negative calcium response element (nCaRE) B2 of the APEX2 promoter together with APEX1. Related references: PMID:11809897
• Functions in neuronal differentiation by modulating alternative splicing of a set of neuronal genes, possibly through interaction with CHD8 and H3K36me3-mediated transcription elongation. Related references: PMID:33174841
• Localizes primarily to the nucleoplasm but can also be found in the cytoplasm, consistent with roles in nuclear RNA processing and cytoplasmic mRNA regulation.
• Undergoes multiple post-translational modifications, including acetylation, methylation, phosphorylation, and ubiquitin-like conjugation (ISGylation), which likely modulate its activity and interactions.
• Predicted molecular weight of approximately 64 kDa, consistent with the observed band in Western blot applications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal domain (aa 282-589) of human HNRNPL, which may include RNA recognition motifs; this design could favor epitope conservation across species, partly explaining the reported human, mouse, and rat cross reactivity.
• For Western blot, the predicted molecular weight is ~64 kDa; consider loading appropriate positive control lysates from human, mouse, or rat cell lines.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), nuclear and cytoplasmic staining is expected; optimize fixation and permeabilization conditions to preserve antigenicity.
• The polyclonal nature may result in batch-to-batch variability; we recommend initial titration and validation in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies for RNA-binding proteins such as HNRNPL. Beyond this polyclonal antibody, we offer matched antibody pairs, optimized monoclonal alternatives, and associated reagents to streamline your splicing and neurobiology assay development. Let our expert team assist in sourcing rare targets and scaling your production needs.