Anti-HNRNPL Polyclonal Antibody for WB, IF/ICC, ELISA - P14866

Core Product Specifications and Parameters

Biological Background: HNRNPL Function and Localization

• HNRNPL is an RNA-binding protein that preferentially interacts with CA-rich elements in pre-mRNAs, enabling its role in alternative splicing regulation.
  Related references: PMID:11809897, PMID:22570490, PMID:24164894

• It functions as a context-dependent splicing regulator, capable of either promoting or inhibiting exon inclusion during mRNA processing.
  Related references: PMID:11809897, PMID:22570490, PMID:24164894

• HNRNPL is a core component of heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and associates with most nascent transcripts produced by RNA polymerase II.
  Related references: PMID:2687284

• Together with APEX1, HNRNPL binds the negative calcium response element (nCaRE) B2 in the APEX2 promoter, linking it to calcium-dependent gene regulation.
  Related references: PMID:11809897

• It participates in a chromatin-associated ribonucleoprotein complex involving ZNF827, HNRNPK, and the circular RNA circZNF827, which negatively regulates neuronal differentiation genes.
  Related references: PMID:33174841

• HNRNPL modulates alternative splicing of essential neuronal genes, likely by mediating H3K36me3-linked transcription elongation and coupling it to co-transcriptional RNA processing via interaction with CHD8.

• Subcellular localization is predominantly nuclear (nucleoplasm), with the ability to shuttle into the cytoplasm under certain conditions.

• The protein undergoes multiple post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination, which may fine-tune its functional interactions.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 282–589 of human HNRNPL, targeting the C-terminal region; this design may detect full-length protein and any isoforms retaining this segment.
• For Western blotting, the predicted molecular weight is ~64 kDa; observe the band pattern and consider extended gel running or phosphatase treatment if additional bands from post-translational modifications are suspected.
• In immunofluorescence/ICC, expect nuclear enrichment with possible nucleoplasmic and weak cytoplasmic signal; optimize permeabilization and fixation conditions for your cell type.
• For ELISA, titrate the antibody to achieve optimal signal-to-noise ratio; consider paired antibody formats if developing a sandwich assay.
• Cross-reactivity is demonstrated with human, mouse, and rat samples; validate species-specific reactivity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement, offering diagnostic manufacturers and researchers a single access point for high-quality antibodies and ancillary reagents. This anti-HNRNPL polyclonal antibody supports gene regulation and neurobiology investigations, complementing our portfolio of validated antibody pairs, bulk monoclonals/polyclonals, and custom sourcing for rare targets. Partner with us to simplify your supply chain from discovery through clinical development.