Anti-HNRNPH1 Polyclonal Antibody for WB - P31943

Core Product Specifications and Parameters

Biological Background: HNRNPH1 Function and Localization

• HNRNPH1 encodes heterogeneous nuclear ribonucleoprotein H, a core component of hnRNP complexes that process pre-mRNA into mature mRNA.
• It functions as a splicing regulator, mediating alternative splicing decisions; for example, it cooperates with CUGBP1 to inhibit inclusion of insulin receptor exon 11.
• The protein binds to RNA, including poly(RG) sequences and the insulin receptor RNA, through its RNA recognition motifs.
• Localized to the nucleoplasm, HNRNPH1 is ubiquitously expressed across human tissues.
• Post-translational modifications include acetylation, methylation, phosphorylation, and ubiquitination, as evidenced by proteomics and direct sequencing.
• Mutations in HNRNPH1 are associated with intellectual disability and neurodevelopmental disorders.
• Keywords: Ribonucleoprotein, RNA-binding, Spliceosome, mRNA processing, mRNA splicing.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is ~49 kDa, but bands may shift due to post-translational modifications; consider optimizing loading controls and antibody dilution for your specific samples.
• In immunohistochemistry (IHC-P), nuclear staining is expected; antigen retrieval and blocking steps should be validated with appropriate tissue controls.
• For immunofluorescence (IF/ICC), permeabilization is essential for nuclear access; titrate the antibody and confirm specificity in knockout or knockdown models.
• The antibody cross-reacts with mouse and rat, making it suitable for studies in these model organisms; nevertheless, verify labeling patterns in the relevant species.
• ELISA can be used for quantitative analysis; include a standard curve and validate the coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to high-quality IVD raw materials, including validated polyclonal antibodies like this anti-HNRNPH1 antibody. Our one-stop platform streamlines procurement of bulk ancillary reagents, optimized antibody pairs, and rare-target sourcing, supporting your work from early development to clinical application. Partner with us to accelerate your pre-mRNA processing and splicing research projects.