Anti-HNRNPDL Polyclonal Antibody for WB, IF/ICC, ELISA - O14979

Core Product Specifications and Parameters

Biological Background: HNRNPDL Function and Localization

• HNRNPDL, also known as JKTBP and laAUF1, is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family, which plays critical roles in RNA metabolism and gene regulation.
• It functions as a transcriptional regulator, capable of both repressing and activating transcription; in differentiated myotubes, it promotes transcription activation (By similarity).
• HNRNPDL binds with high affinity to AU-rich elements (AREs) in the 3'-UTR of mRNAs, including those of proto-oncogenes and cytokines, and also interacts with double- and single-stranded DNA sequences.
• The protein localizes to both the nucleus and cytoplasm, reflecting its dual roles in transcriptional control and cytoplasmic mRNA regulation.
• It is widely expressed in human tissues, including heart, brain, skeletal muscle, and various cancer cell lines, with implications in developmental and disease processes.
• Post-translational modifications include acetylation, methylation, and phosphorylation, and it is subject to ubiquitin-like conjugation; mutations in HNRNPDL are associated with limb-girdle muscular dystrophy (LGMD1G/LGMDD3).
• Keywords from UniProt highlight its involvement in alternative splicing, transcription regulation, and DNA/RNA binding, underscoring its multifunctional nature.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 261–420 of human HNRNPDL, a region that may be conserved across species; consider validating cross-reactivity in your specific experimental system.
• For Western blot, a band around the predicted molecular weight of 46 kDa is expected; we recommend using appropriate positive control lysates from human, mouse, or rat tissues or cell lines.
• In immunofluorescence and immunocytochemistry, HNRNPDL staining is anticipated in both nuclear and cytoplasmic compartments; fixation and permeabilization protocols should be optimized for your cell type.
• The antibody is suitable for ELISA; titration experiments are advised to determine optimal coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw material provider, CamelBio offers streamlined access to validated antibodies, recombinant proteins, and ancillary reagents for your diagnostic and research needs. This anti-HNRNPDL antibody supports studies in transcriptional regulation and RNA-binding protein biology, and our platform can source additional reagents for rare targets and bulk requirements.