Anti-HNRNPD Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14103

Core Product Specifications and Parameters

Biological Background: HNRNPD Function and Localization

• HNRNPD, also known as AUF1, is a heterogeneous nuclear ribonucleoprotein that specifically binds AU-rich elements (AREs) in the 3'-UTR of many labile mRNAs, such as proto-oncogenes and cytokines, to regulate mRNA stability and turnover.
• It functions not only in RNA metabolism but also as a transcription factor by binding to double- and single-stranded DNA, possibly influencing telomere maintenance through interaction with TTAGGG repeats.
• The protein contains two RNA recognition motifs (RRMs) that recognize the 5'-UUAG-3' motif in RNA and can bind both RNA and single-stranded telomeric DNA, with RRM1 inhibiting DNA quadruplex formation.
• HNRNPD is implicated in cytoplasmic deadenylation/decay processes, including FOS mRNA degradation mediated by the major coding-region determinant of instability, and may facilitate translationally coupled mRNA turnover.
• It plays a role in the circadian clock by directly binding the 3'UTR of CRY1 mRNA and enhancing its rhythmic translation, and may also modulate PER2 translation.
• Multiple isoforms are generated through alternative splicing, giving rise to variants such as p37, p40, p42, and p45, which exhibit different activities in mRNA decay.
• The protein shuttles between the nucleus and cytoplasm, with its subcellular localization affecting its functional role in RNA processing and degradation.
• Post-translational modifications include acetylation, methylation, phosphorylation, and ubiquitination, which can modulate its binding affinity and subcellular distribution.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein corresponding to amino acids 1-306 of human HNRNPD (full-length), thus the antibody is expected to recognize both nuclear and cytoplasmic pools as well as major isoforms.
• For western blot (WB), an expected band around 38 kDa is detected in human, mouse, and rat lysates; however, isoform-specific patterns may vary, so validation in your specific experimental system is advised.
• This polyclonal antibody can be applied in immunohistochemistry (IHC-P), immunofluorescence (IF/ICC), and ELISA; consider optimizing fixation/permeabilization conditions for IF to preserve epitope accessibility.
• When studying circadian-related expression, time-series sampling and appropriate positive controls (e.g., synchronized cell lines) are recommended to capture rhythmic changes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with a comprehensive selection of high-quality IVD raw materials. This anti-HNRNPD polyclonal antibody supports research into mRNA regulation, cancer biology, and circadian rhythm mechanisms, and CamelBio can further assist you in sourcing validated antibody pairs, bulk ancillary reagents, and other rare-target raw materials. Our expert team is ready to streamline your supply chain from discovery through clinical application.