Anti-HNRNPD Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q14103

Core Product Specifications and Parameters

Biological Background: HNRNPD Function and Localization

• Heterogeneous nuclear ribonucleoprotein D0 (HNRNPD), also known as AU-rich element RNA-binding protein 1 (AUF1), is encoded by the HNRNPD gene. Multiple isoforms are produced by alternative splicing.
• HNRNPD/AUF1 binds with high affinity to AU-rich elements (AREs) in the 3'-UTR of many proto-oncogene and cytokine mRNAs, thereby regulating mRNA stability and translation.
• The protein also functions as a transcription factor by binding to double- and single-stranded DNA, including telomeric repeats, and may influence telomere elongation by inhibiting DNA quadruplex formation.
• HNRNPD is involved in translationally coupled mRNA turnover and mediates the cytoplasmic deadenylation/translational decay interplay of FOS mRNA via the major coding-region determinant of instability (mCRD) domain.
• It plays a role in circadian rhythm regulation by directly binding to the 3'UTR of CRY1 mRNA and inducing rhythmic translation of CRY1, and may also regulate PER2 translation.
• Subcellular localization is both nuclear and cytoplasmic, consistent with its roles in transcription, mRNA processing, and translational control.
• Post-translational modifications include acetylation, methylation, phosphorylation, and ubiquitination, which likely modulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–280) encompassing the RNA recognition motifs (RRMs). This design may recognize multiple HNRNPD isoforms.
• For Western blot (WB), the expected band is approximately 38 kDa. Optimize sample preparation and blocking conditions for detection in relevant cell lines.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate nuclear and cytoplasmic staining patterns using appropriate controls.
• For immunoprecipitation (IP) and ELISA, it is advisable to titrate the antibody and optimize buffer conditions per the specific assay and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs and optimized monoclonal antibodies for critical targets like HNRNPD/AUF1. Our comprehensive portfolio supports mRNA stability studies, circadian rhythm research, and oncology applications. From bulk ancillary reagents to rare target sourcing, we bridge the gap from concept to clinic with reliable, scalable supply solutions.