Anti-HNRNPA3 Rabbit Polyclonal Antibody for WB - P51991

Core Product Specifications and Parameters

Biological Background: HNRNPA3 Function and Localization

• HNRNPA3 is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family, which are major nuclear RNA-binding proteins involved in RNA processing and metabolism.
• It plays a role in cytoplasmic trafficking of RNA, specifically by binding to the cis-acting response element A2RE (hnRNP A2 response element) in target mRNAs.
• HNRNPA3 may be involved in pre-mRNA splicing, contributing to the regulation of alternative splicing events.
• The protein is localized primarily in the nucleus, consistent with its role in RNA processing, but can also shuttle between nucleus and cytoplasm for RNA transport.
• Post-translational modifications include acetylation, methylation, phosphorylation, and ubiquitination, which may regulate its function and subcellular localization.
• Alternative splicing of the HNRNPA3 gene produces multiple isoforms, adding to functional diversity.
• It contains RNA recognition motifs (RRMs) and is part of ribonucleoprotein complexes and spliceosomes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to an internal region (aa 100-200) of human HNRNPA3. For immunodetection, consider using whole cell lysates or nuclear extracts expected to express the target at ~40 kDa.
• The antibody is validated for Western blot (WB), immunohistochemistry (IHC-P), and ELISA. For WB, typical dilution ranges for polyclonal antibodies are 1:500-1:2000; optimization is recommended.
• With cross-reactivity to mouse and rat, this antibody is suitable for analyzing HNRNPA3 in rodent tissues and cell lines. Confirm specificity with appropriate controls and database-predicted molecular weight.
• Since HNRNPA3 may undergo post-translational modifications, observe for potential band shifts in WB and consider treatment with dephosphorylating enzymes for verification.
• For IHC-P, antigen retrieval may be required; test different protocols to achieve optimal staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing for RNA biology research. For HNRNPA3 and beyond, we help bridge your procurement needs with reliable supply solutions.