Anti-HNRNPA0 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q13151

Core Product Specifications and Parameters

Biological Background: HNRNPA0 Function and Localization

• Heterogeneous nuclear ribonucleoprotein A0 (HNRNPA0, also known as HNRPA0) is an mRNA-binding protein and a component of ribonucleosomes.
• It specifically binds AU-rich element (ARE)-containing mRNAs, playing a critical role in post-transcriptional regulation.
• Involved in the regulation of cytokine mRNAs, influencing their stability and translation to control inflammatory responses.
• Localizes to the nucleus, consistent with its function in mRNA processing and assembly of ribonucleoprotein complexes.
• Undergoes multiple post-translational modifications, including acetylation, methylation, phosphorylation, isopeptide bond formation, and ubiquitination, which may modulate its RNA-binding activity and interactions.
• Contains RNA recognition motif repeats essential for target mRNA binding and regulatory functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–180) of human HNRNPA0, which is highly conserved across human, mouse, and rat, supporting broad species reactivity.
• For Western blot (WB), the predicted molecular weight is approximately 31 kDa; consider using appropriate loading controls and validating band specificity in your sample system.
• In immunoprecipitation (IP) and ELISA, optimize antibody concentration and ensure proper negative controls to confirm specific binding, especially when working with endogenous HNRNPA0.
• Although the antibody is validated for human, mouse, and rat, cross-reactivity in other species may be possible due to the conserved immunogen region; validate in the relevant species if needed.
• Store the antibody as recommended (shipped on ice bags) and avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated access to IVD raw materials, supporting diagnostic manufacturers and researchers from early development to clinical application. For post-transcriptional regulation and cytokine research, we supply validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our one-stop sourcing also includes rare target raw material procurement, ensuring you have the critical tools needed for assay development and biomarker studies.