Anti-hnRNP U Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q00839

Core Product Specifications and Parameters

Biological Background: hnRNP U Function and Localization

• hnRNP U is a multifunctional DNA- and RNA-binding protein that organizes chromatin architecture by binding to chromatin-associated RNAs (caRNAs) and scaffold/matrix attachment region (S/MAR) elements, essential for maintaining genomic stability. Related references: PMID:1324173, PMID:8174554, PMID:10671544, PMID:11003645, PMID:11909954, PMID:28622508
• It acts as a transcriptional regulator by promoting pre-initiation complex assembly through TFIIH interaction, while also inhibiting elongation by blocking CTD phosphorylation of RNA Pol II. Related references: PMID:10490622, PMID:15711563
• hnRNP U is crucial for X-chromosome inactivation by directly binding and anchoring Xist long non-coding RNA to the inactive X chromosome.
• The protein modulates alternative splicing by regulating U2 snRNP biogenesis and binding to G/U-rich regions in pre-mRNAs, including SMN1/2. Related references: PMID:22325991
• It regulates mRNA stability, enhancing expression of targets like MYC and TNFA through interactions with the CRD-mediated complex and 3'-UTRs. Related references: PMID:17174306, PMID:17289661, PMID:19029303
• During mitosis, phosphorylation at Ser-59 by PLK1 is required for proper chromosome alignment and segregation, and it contributes to spindle organization. Related references: PMID:21242313, PMID:25986610
• hnRNP U is predominantly nuclear, but also localizes to cytoskeletal structures including centrosomes, kinetochores, spindle poles, and midbody during cell division.

Experimental Guidance and Technical Tips

• Given the immunogen design (C-terminal fragment, a.a. 608-825), this polyclonal antibody is expected to detect full-length hnRNP U as well as potential splice variants containing this region.
• For Western blotting, an ~91 kDa band is anticipated; use whole-cell or nuclear extracts from human, mouse, or rat tissues/cells as positive controls.
• When performing immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization conditions to preserve nuclear architecture, as hnRNP U displays both nuclear and mitotic apparatus localization.
• For immunohistochemistry (IHC-P), antigen retrieval may be necessary; validate with known positive and negative tissue samples.
• For immunoprecipitation (IP), consider using nuclear lysates and validate co-precipitated nucleic acids or protein partners if studying chromatin/RNA interactions.
• Species cross-reactivity with mouse and rat has been reported, but always verify reactivity in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials, including this hnRNP U antibody, validated for multiple applications and species reactivity to support chromatin, transcription, and RNA biology research. As a one-stop sourcing partner, we offer optimized monoclonal/polyclonal antibodies, recombinant proteins, and ancillary bulk reagents that streamline assay development from concept to clinic. Contact us for custom formulations, technical consulting, and reliable supply of rare-target raw materials tailored to your diagnostic needs.