Anti-hnRNP U Monoclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - Q00839

Core Product Specifications and Parameters

Biological Background: Heterogeneous nuclear ribonucleoprotein U Function and Localization

• hnRNP U (also known as SAF-A, p120, GRIP120) is a member of the heterogeneous nuclear ribonucleoprotein family, encoded by the HNRNPU gene.
• It regulates interphase large-scale gene-rich chromatin organization through chromatin-associated RNAs (caRNAs), thereby maintaining genomic stability. Related references: PMID:1324173 PMID:28622508 PMID:8174554
• Functions as a dual regulator of RNA polymerase II transcription, promoting initiation via the TFIIH complex and blocking elongation by inhibiting CTD phosphorylation. Related references: PMID:10490622 PMID:15711563 PMID:19617346
• Modulates pre-mRNA alternative splicing by regulating U2 snRNP biogenesis and enhances mRNA stability of targets such as MYC and TNFA. Related references: PMID:22325991 PMID:17174306 PMID:19029303
• Participates in mitotic spindle organization, kinetochore attachment, and chromosome congression; phosphorylation at Ser-59 by PLK1 is critical for orderly mitosis. Related references: PMID:21242313 PMID:25986610
• Localizes to the nucleus, nuclear matrix, chromosomes, centrosomes, spindle microtubules, and midbody, reflecting its diverse roles in interphase and mitosis.
• Widely expressed across tissues, underscoring its fundamental importance in RNA metabolism and chromatin regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1‑100) of human hnRNP U; consider verifying antibody specificity for the full-length protein and any relevant isoforms in your assay system.
• Given the indicated applications (WB, IP, IF/ICC, IHC-P, ELISA), optimization of antibody concentration and detection protocols for each technique is recommended.
• For immunofluorescence, note that hnRNP U localization can be both nuclear and cytoplasmic, particularly in mitotic cells; proper fixation and permeabilization conditions are essential.
• Cross-reactivity with mouse and rat has been reported, but validation in your specific model system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your strategic partner for IVD raw material procurement, offering a comprehensive portfolio that includes this anti-hnRNP U antibody and related molecular biology reagents. Whether you need validated antibody pairs, bulk ancillary reagents, or assistance sourcing rare targets, our global supply network ensures reliable access for your diagnostic and research needs. From early-stage development to clinical validation, CamelBio supports your workflow with a one-stop solution tailored to the demands of the IVD industry.