Anti-hnRNP K Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P61978

Core Product Specifications and Parameters

Biological Background: hnRNP K Function and Localization

• hnRNP K is a major pre-mRNA-binding protein with high affinity for poly(C) sequences, playing a central role in hnRNA metabolism, splicing, and mRNA processing.
• It is a critical mediator of the p53/TP53 DNA damage response, functioning as both a transcriptional coactivator (when sumoylated) for genes like p21/CDKN1A and a repressor through interactions with non-coding RNAs such as lincRNA-p21, thereby regulating apoptosis and cell cycle arrest.
• As part of a ribonucleoprotein complex containing ZNF827, HNRNPL, and circZNF827, hnRNP K negatively regulates the transcription of genes involved in neuronal differentiation (PubMed:33174841). Related references: PMID:33174841
• The protein dynamically localizes to multiple subcellular compartments: nucleus, nucleoplasm, cytoplasm, and podosomes, consistent with its roles in RNA processing, signaling, and cell adhesion.
• hnRNP K undergoes extensive post-translational modifications, including phosphorylation, acetylation, methylation, sumoylation, and glycosylation, which modulate its activity, localization, and interaction networks.
• It possesses single-stranded DNA-binding activity and is implicated in host-virus interactions, highlighting its multifaceted contributions to cellular homeostasis and pathogenesis.
• Alternative splicing of the HNRNPK pre-mRNA generates multiple isoforms, though their functional distinctions are not fully characterized here.
• Keywords from UniProt include: mRNA processing, spliceosome, transcription regulation, activator, repressor, and intellectual disability, underscoring its broad regulatory roles in gene expression and disease.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 1-100 of human hnRNP K; sequence alignment with mouse and rat orthologs shows significant homology, supporting the reported cross-reactivity. Validate specificity in your target species and tissue.
• In Western blot (WB), a predominant band around 49-51 kDa is expected; however, due to extensive post-translational modifications, multiple bands or slight mass shifts may be observed. Use appropriate molecular weight markers and positive controls (e.g., HeLa or HEK293 whole-cell lysates).
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), analyze subcellular localization patterns: expect both nuclear and cytoplasmic staining. Optimize antigen retrieval (e.g., citrate buffer pH 6.0) and permeabilization steps. Consider using podosome markers for co-localization studies.
• In ELISA applications, the antibody may serve as a capture or detection reagent; however, assay conditions (coating buffer, blocking, wash steps) and antibody pairing must be empirically determined.
• Given hnRNP K's involvement in p53 signaling, evaluating expression changes upon DNA damage induction (e.g., by doxorubicin or UV treatment) can serve as a functional validation strategy.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop platform serving diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, technical services, and consultative support—from concept to clinic. Our anti-hnRNP K rabbit monoclonal antibody exemplifies the reliable, application-validated reagents we offer for studying RNA biology, cancer mechanisms, and neurodevelopment. Beyond individual antibodies, we provide matched antibody pairs, bulk ancillary reagents, and tailored sourcing solutions for rare or specialized targets, ensuring your projects stay on track with seamless supply and expert guidance.