Anti-hnRNP K Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P61978

Core Product Specifications and Parameters

Biological Background: hnRNP K Function and Localization

• hnRNP K (Heterogeneous nuclear ribonucleoprotein K), also known as Transformation up-regulated nuclear protein (TUNP), is a major pre-mRNA-binding protein with high affinity for cytidine-rich sequences. It plays a critical role in pre-mRNA metabolism, including alternative splicing and mRNA processing.
• In the DNA damage response, hnRNP K acts as a dual regulator of p53/TP53 signaling, functioning as a sumoylation-dependent transcriptional coactivator for p21/CDKN1A and 14-3-3 sigma/SFN induction, and as a repressor via interaction with the long non-coding RNA lincRNA-p21 to promote apoptosis.
• hnRNP K participates in a ribonucleoprotein complex containing ZNF827, HNRNPL, and the circular RNA circZNF827, which associates with chromatin and may negatively regulate neuronal differentiation genes. Related references: PMID:33174841
• Localizes to the nucleus (nucleoplasm), cytoplasm, and cell projections such as podosomes, supporting its dynamic shuttling and diverse roles.
• Undergoes various post-translational modifications including acetylation, methylation, phosphorylation, and UBL conjugation (sumoylation), which modulate its activity and interactions.
• In addition to RNA binding, hnRNP K can bind poly(C) single-stranded DNA and is implicated in host-virus interactions and intellectual disability when mutated.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-280) conserved across human, mouse, and rat, supporting the observed cross-reactivity. Consider sequence alignment validation for other species if needed.
• For Western blot (WB), a band near 51 kDa is expected; nuclear fractionation may enhance detection due to hnRNP K’s enrichment in the nucleus.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), assess expression patterns in positive control tissues or cell lines, and optimize antigen retrieval and permeabilization conditions.
• For ELISA applications, validate binding using recombinant hnRNP K or lysates, and titrate the antibody for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with integrated access to high-quality IVD raw materials, streamlining development from concept to clinic. For hnRNP K research in RNA processing and DNA damage, we offer tailored sourcing solutions including validated polyclonal antibodies, bulk ancillary reagents, and custom options to meet your assay needs.