Anti-hnRNP F Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P52597

Core Product Specifications and Parameters

Biological Background: hnRNP F Function and Localization

• hnRNP F is a component of heterogeneous nuclear ribonucleoprotein (hnRNP) complexes, which provide the substrate for pre-mRNA processing events prior to their translation in the cytoplasm.
• It plays a key role in the regulation of alternative splicing by binding G-rich sequences in pre-mRNAs and maintaining target RNA in an unfolded state.
• Subcellularly localized in the nucleus, specifically the nucleoplasm.
• Ubiquitously expressed across tissues.
• Subject to post-translational modifications including acetylation, phosphorylation, and ubiquitination, as identified by proteomics approaches.
• Contains RNA recognition motifs (RRMs) and belongs to a family of RNA-binding proteins involved in mRNA processing and splicing.

Experimental Guidance and Technical Tips

• Since the immunogen is a synthetic peptide derived from human hnRNP F, confirm sequence homology if cross-reactivity with mouse or rat is critical for your experiment.
• For Western blotting, expect a band at ~46 kDa; validate detection in appropriate positive lysates and consider loading controls.
• In immunofluorescence/immunocytochemistry, a nuclear staining pattern is anticipated; optimize fixation, permeabilization, and use a nuclear counterstain.
• For ELISA, titration of the antibody against the peptide immunogen or recombinant hnRNP F is recommended to determine the optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, offering this hnRNP F monoclonal antibody alongside validated antibody pairs, bulk ancillary reagents, and rare target sourcing solutions. We support diagnostic manufacturers and researchers in advancing RNA processing and alternative splicing studies from concept to clinic.