Anti-hnRNP E2/PCBP2 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q15366

Core Product Specifications and Parameters

Biological Background: hnRNP E2/PCBP2 Function and Localization

• Poly(rC)-binding protein 2 (PCBP2), also known as hnRNP E2 or alpha-CP2, is a member of the hnRNP family with multiple isoforms generated through alternative splicing. It is ubiquitously expressed across human tissues.
• Functions as a single-stranded nucleic acid binding protein with high affinity for oligo dC and poly(rC) tracts; also binds poly(rU).
  Related references:
  PMID:12414943
  PMID:7607214
• Serves as a negative regulator of innate antiviral signaling: bridges mitochondrial antiviral-signaling protein (MAVS) and the E3 ubiquitin ligase ITCH, triggering MAVS ubiquitination and degradation, thereby dampening MAVS-mediated responses.
  Related references:
  PMID:19881509
• Additionally inhibits the cGAS-STING pathway by directly interacting with CGAS and preventing its phase separation into active liquid-like droplets.
  Related references:
  PMID:35322803
• Cooperates with its paralog PCBP1 to support erythropoiesis, likely through regulating mRNA splicing (by similarity).
• During poliovirus infection, PCBP2 binds the viral internal ribosome entry site (IRES) to stimulate IRES-mediated translation and collaborates with the viral 3CD protein to initiate viral RNA replication.
  Related references:
  PMID:12414943
  PMID:24371074
• Subcellular localization is both nuclear and cytoplasmic, consistent with its roles in RNA metabolism and signaling regulation.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of PCBP2 is approximately 38.6 kDa (UniProt), but the antibody typically detects a band around 39 kDa. Consider using whole-cell lysates from human (e.g., HeLa, HEK293), mouse, or rat cell lines and validate with appropriate controls.
• The immunogen corresponds to amino acids 220–350, a region likely conserved across species; the antibody is reported to cross-react with human, mouse, and rat samples. Perform necessary validation in your experimental system.
• For immunoprecipitation, the antibody’s target region may be accessible in native conformations; optimize lysis and washing conditions to preserve protein interactions.
• When using ELISA, consider pairing with a detection antibody against a distinct epitope to achieve sensitive quantification; verify specificity against recombinant protein.
• Due to alternative splicing and potential post-translational modifications, multiple bands may appear; confirm by knockdown or mass spectrometry if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive range of IVD raw materials for manufacturers and research labs, including validated monoclonal antibodies like this anti-PCBP2 clone, optimized for WB, IP, and ELISA. Beyond individual reagents, we supply matched antibody pairs, bulk ancillary reagents, and can assist in sourcing rare targets across the IVD development pipeline—from concept to clinic. With expertise in RNA biology and antiviral research, we are your reliable partner for diagnostic raw-material procurement.