Anti-hnRNP E2/PCBP2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q15366

Core Product Specifications and Parameters

Biological Background: hnRNP E2/PCBP2 Function and Localization

• Encoded by the PCBP2 gene, this protein is also known as hnRNP E2, alpha-CP2, and heterogeneous nuclear ribonucleoprotein E2.
• Functions as a single-stranded nucleic acid binding protein with high affinity for oligo dC and poly(rC), serving as a major cellular poly(rC)-binding protein; also binds poly(rU). Related references: PMID:12414943, PMID:7607214
• Negatively regulates antiviral signaling by mediating MAVS ubiquitination and degradation via the E3 ligase ITCH, and by suppressing the cGAS-STING pathway through interaction with CGAS to prevent its activation. Related references: PMID:19881509, PMID:35322803
• Together with PCBP1, contributes to erythropoiesis, possibly through regulation of mRNA splicing.
• During poliovirus infection, binds the viral internal ribosome entry site (IRES) to stimulate IRES-mediated translation and participates in viral RNA replication with protein 3CD. Related references: PMID:12414943, PMID:24371074
• Localizes to both the nucleus and cytoplasm, and is detected in all tissues examined.
• Classified under keywords including antiviral defense, innate immunity, RNA-binding, and host-virus interaction; undergoes phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 150-250 of human PCBP2. For immunoprecipitation, consider that this region may be inaccessible in certain protein conformations.
• In Western blot, a specific band near 39 kDa is expected; additional bands may appear due to alternative splicing or post-translational modifications.
• For IHC-P and IF/ICC, optimize antigen retrieval and permeabilization protocols to detect both nuclear and cytoplasmic localizations.
• Validate antibody specificity in the relevant sample system, such as by using knockout or knockdown controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to validated antibody pairs, optimized antibodies, and bulk ancillary reagents for IVD development. For hnRNP E2/PCBP2, we offer this polyclonal antibody along with related raw materials to support studies on RNA processing, viral host interactions, and innate immunity. Our global supply chain ensures seamless procurement from concept to clinic.