Anti-hnRNP E1/PCBP1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q15365

Core Product Specifications and Parameters

Biological Background: hnRNP E1/PCBP1 Function and Localization

• Protein identity: PCBP1, also known as hnRNP E1, alpha-CP1, is a member of the poly(rC)-binding protein family.
• Binds single-stranded nucleic acids, with a preference for oligo dC, implicating it in mRNA stability, translation, and splicing regulation. Related references: PMID:15731341, PMID:7556077, PMID:7607214
• Together with PCBP2, required for erythropoiesis, possibly by regulating mRNA splicing.
• Plays a role in initiation of viral RNA replication upon poliovirus infection in concert with viral protein 3CD.
• Subcellular localization: Found in both nucleus and cytoplasm.
• Tissue specificity: Abundantly expressed in skeletal muscle, thymus, and peripheral blood leukocytes; lower expression in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal, and thyroid glands.
• Contains KH domains, characteristic of RNA-binding proteins, and undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide between amino acids 100-200; the antibody may detect endogenous PCBP1 at ~37 kDa by WB. Consider using human or mouse cell lysates.
• For IF/ICC, optimize fixation and permeabilization conditions to preserve antigenicity.
• ELISA: suitable for direct detection of the immunogen peptide or native protein.
• Validate antibody specificity using knockout or siRNA controls in relevant cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for research into RNA-binding proteins like hnRNP E1/PCBP1. Whether you need rare target antibodies or technical consulting, we streamline procurement from concept to clinic.