Anti-hnRNP E1/PCBP1 Rabbit Monoclonal Antibody for WB, ELISA - Q15365

Core Product Specifications and Parameters

Biological Background: PCBP1 Function and Localization

• PCBP1 (Poly(rC)-binding protein 1), also known as hnRNP E1, Alpha-CP1, or SUB2.3, is encoded by the PCBP1 gene.
• It functions as a single-stranded nucleic acid binding protein with a high affinity for oligo dC stretches. Related references: PMID:15731341 PMID:7556077 PMID:7607214
• Together with PCBP2, it is required for erythropoiesis, likely by modulating mRNA splicing (By similarity).
• In the context of poliovirus infection, PCBP1 participates in the initiation of viral RNA replication in conjunction with the viral 3CD protein.
• The protein localizes to both the nucleus and cytoplasm.
• PCBP1 is abundantly expressed in skeletal muscle, thymus, and peripheral blood leukocytes, with lower expression in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal gland, and thyroid gland.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination; it also contains RNA-binding KH domains.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the N-terminal region (aa 1–100) of human PCBP1; thus, the antibody may recognize epitopes in that domain.
• For Western blot (WB), consider standard protocols with whole-cell or tissue lysates from human, mouse, or rat samples, as cross-reactivity has been reported for these species.
• In ELISA applications, optimize coating antigen concentrations and detection conditions.
• As a rabbit monoclonal antibody, it offers high specificity and lot-to-lot consistency; nonetheless, validate performance in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined, one-stop sourcing solution for IVD raw materials, including validated monoclonal antibodies like this anti-PCBP1 antibody, which supports RNA biology and infectious disease research. Our supply capabilities encompass optimized antibody pairs, bulk ancillary reagents, and custom raw-material sourcing to bridge the gap from concept to clinical application.