Anti-hnRNP C1/C2 Rabbit Polyclonal Antibody - P07910

Core Product Specifications and Parameters

Biological Background: hnRNP C Function and Localization

• HNRNPC produces two major isoforms (C1 and C2) via alternative splicing.
• Binds pre-mRNA and nucleates the assembly of 40S hnRNP particles; single tetramers cover 230‑240 nucleotides, while trimers of tetramers bind ~700 nucleotides. Related references: PMID:8264621
• Interacts with poly‑U tracts in the 3'‑UTR or 5'‑UTR of mRNAs, modulating stability and translational control. Related references: PMID:12509468, PMID:16010978, PMID:7567451
• May participate in early spliceosome assembly and pre‑mRNA splicing.
• N6‑methyladenosine (m6A) induces a structural switch that facilitates HNRNPC binding, thereby regulating splicing decisions. Related references: PMID:25719671
• Localizes to the nucleus.
• Post‑translational modifications include acetylation, phosphorylation, and ubiquitination.
• Mutations in HNRNPC are associated with intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 86‑190 of human hnRNP C; this central region may recognize both C1 and C2 isoforms.
• For western blot, a band near 34 kDa is expected; consider standard reducing conditions and validate in representative cell lines.
• For immunohistochemistry, antigen retrieval may be required; optimize fixation and blocking for the tissue of interest.
• In immunofluorescence, permeabilization is essential for nuclear detection; co‑staining with a nuclear marker can confirm localization.
• Cross‑reactivity with mouse and rat is reported; verify performance in the species‑specific sample system to ensure consistent results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated procurement partner for IVD raw materials, offering validated hnRNP C antibodies and complementary reagents to accelerate mRNA processing research. From optimized monoclonal/polyclonal antibodies and matched antibody pairs to bulk ancillary reagents and rare‑target sourcing, we provide one‑stop access to the tools needed to move from concept to clinic.