Anti-hnRNP A2B1 Rabbit Monoclonal Antibody [KD Validated] for WB, IHC-P, IF/ICC, ELISA - P22626

Core Product Specifications and Parameters

Biological Background: hnRNP A2B1 Function and Localization

• Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2B1) is an RNA-binding protein that packages nascent pre-mRNAs into hnRNP particles, condensing and stabilizing transcripts to prevent tangling and knotting. Related references: PMID:19099192
• hnRNP A2B1 specifically recognizes the A2RE sequence motif present on certain mRNAs, mediating their transport to the cytoplasm in oligodendrocytes and neurons, and also facilitating HIV-1 genomic RNA trafficking. Related references: PMID:10567417
• It acts as a nuclear 'reader' of N6-methyladenosine (m6A), binding m6A-containing primary miRNAs to enhance their processing by DGCR8, and may similarly regulate pre-mRNA splicing. Related references: PMID:26321680
• Sumoylated hnRNP A2B1 is involved in sorting miRNAs into exosomes, potentially through recognition of m6A-modified pre-miRNAs. Related references: PMID:24356509
• The protein regulates alternative splicing of PKM, repressing exon 9 inclusion to favor the PKM2 isoform, which is critical in tumor metabolism. Related references: PMID:20010808
• Upon sensing nuclear viral DNA, hnRNP A2B1 homodimerizes, undergoes JMJD6-mediated demethylation, and translocates to the cytoplasm to activate the TBK1-IRF3 pathway, leading to interferon production. Related references: PMID:31320558
• It localizes to the nucleus, nucleoplasm, and cytoplasm, and is secreted via exosomes; post-translational modifications include acetylation, phosphorylation, methylation, and ubiquitination.
• Alternative splicing produces multiple isoforms, with the B1 isoform containing an additional N-terminal domain.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 151-250) of human hnRNP A2B1. For Western blot, a band at approximately 37 kDa is expected under reducing conditions; however, post-translational modifications may cause migration shifts.
• For immunohistochemistry, perform antigen retrieval (e.g., heat-induced epitope retrieval in citrate buffer) due to the nuclear localization; use known positive tissues like brain or testis.
• In immunofluorescence/ICC, ensure permeabilization with Triton X-100 or similar to detect both nuclear and cytoplasmic pools.
• The antibody cross-reacts with mouse and rat, so it is applicable to rodent model studies; always include relevant positive/negative controls.
• For ELISA, optimize coating concentrations and use recombinant protein or cell lysates as targets; a sandwich pair may be developed with another hnRNP A2B1 antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated monoclonal and polyclonal antibodies, antigen pairs, and ancillary reagents. Our hnRNP A2B1 antibody supports research into mRNA metabolism, viral infection mechanisms, and cancer biology, facilitating diagnostic assay development. For bulk orders, customized sourcing, or technical assistance, reach out to our dedicated support team.