Anti-hnRNP A1 Rabbit Monoclonal Antibody for WB - P09651

Core Product Specifications and Parameters

Biological Background: hnRNP A1 Function and Localization

• hnRNP A1 is a multifunctional RNA-binding protein that packages pre-mRNA into heterogeneous nuclear ribonucleoprotein (hnRNP) particles, transports poly(A) mRNA from the nucleus to the cytoplasm, and modulates splice site selection. (PubMed:17371836)
  Related references: PMID:17371836
• It represses the inclusion of exon 9 in the pyruvate kinase PKM transcript by binding to flanking sequences, promoting the production of the PKM M2 isoform, a key regulator of cancer metabolism. (PubMed:20010808)
  Related references: PMID:20010808
• hnRNP A1 binds to the internal ribosome entry site (IRES) of APAF1 mRNA and inhibits its translation, suppressing the apoptosis pathway. (PubMed:31498791)
  Related references: PMID:31498791
• The protein may also interact with specific miRNA hairpins, suggesting a role in miRNA-mediated gene regulation. (PubMed:28431233)
  Related references: PMID:28431233
• During viral infection, hnRNP A1 can be hijacked to facilitate HCV RNA replication, and cleavage by Enterovirus 71 protease 3C leads to increased APAF1 translation and apoptosis.
• hnRNP A1 dynamically shuttles between the nucleus and cytoplasm, where its functions are regulated by numerous post-translational modifications, including methylation, phosphorylation, acetylation, and ubiquitination.
• Mutations in the HNRNPA1 gene are associated with amyotrophic lateral sclerosis (ALS19, ALS20) and multisystem proteinopathy (IBMPFD3), implicating the protein in neurodegenerative disease.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal glycine-rich domain (amino acids 250–350). This region is relatively conserved; verify recognition in your species/organism of interest if not human, mouse, or rat.
• For Western blot, a band at approximately 39 kDa is expected. Consider using siRNA-mediated knockdown or knockout cell lines to confirm band specificity.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and blocking steps, as hnRNP A1 is a nuclear protein and may require permeabilization.
• The antibody cross-reacts with mouse and rat, enabling its use in rodent tissue studies. However, proper controls are recommended when extrapolating to other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, serving diagnostic manufacturers, labs, and research institutes worldwide. For hnRNP A1-related studies in mRNA processing and neurodegeneration, we can supply validated monoclonal antibodies, recombinant antigens, and bulk ancillary reagents to streamline your assay development. Whether you need ready-to-use antibody pairs or custom conjugation services, CamelBio bridges the gap from concept to clinic.