Anti-hnRNP A1 Polyclonal Antibody for WB, IF/ICC, ELISA - P09651

Core Product Specifications and Parameters

Biological Background: hnRNP A1 Function and Localization

• Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1), also known as helix-destabilizing protein or single-strand RNA-binding protein, is an RNA-binding protein encoded by the HNRNPA1 gene (Gene ID: 3178).
• Involved in packaging pre-mRNA into hnRNP particles, transporting poly(A) mRNA from nucleus to cytoplasm, and modulating splice site selection. Related references: PMID:17371836
• Plays a role in splicing of pyruvate kinase PKM by repressing sequences flanking PKM exon 9, inhibiting exon 9 inclusion, leading to exon 10 inclusion and production of the PKM M2 isoform. Related references: PMID:20010808
• Binds to the IRES and inhibits translation of apoptosis protease activating factor APAF1. Related references: PMID:31498791
• May bind to specific miRNA hairpins. Related references: PMID:28431233
• Localizes to both nucleus and cytoplasm, participating in nucleocytoplasmic shuttling of mRNA.
• Undergoes acetylation, methylation, phosphorylation, and ubiquitin conjugation; disease variants are associated with amyotrophic lateral sclerosis (ALS19, ALS20) and neurodegeneration.
• May play a role in HCV RNA replication; cleavage by Enterovirus 71 protease 3C leads to increased APAF1 translation and apoptosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1‑140) of human hnRNP A1; the antibody may recognize full-length and N-terminal fragments. KO validation supports specificity in western blotting.
• For western blotting, use whole-cell or tissue lysates from human, mouse, or rat. Include appropriate controls and validate detection of the ~39 kDa band.
• For immunofluorescence/ICC, expect nuclear and cytoplasmic staining. Optimize fixation and permeabilization conditions for the relevant cell line.
• For ELISA, test against recombinant hnRNP A1 protein or cell lysates; confirm linearity and detection limit in the experimental system.
• Consider cross-reactivity with other species if needed, and validate by sequence homology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD-grade raw materials and technical support. For projects targeting hnRNP A1—a key player in mRNA regulation and cancer biology—we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to accelerate assay development. Our sourcing network also covers rare targets and custom immunogen design, ensuring reliable supply from research to clinical application.