Anti-HNMT Rabbit Polyclonal Antibody for WB, IP, ELISA - P50135

Core Product Specifications and Parameters

Biological Background: HNMT Function and Localization

• HNMT (Histamine N-methyltransferase) is the primary enzyme responsible for the inactivation of histamine through N-methylation, converting histamine to Nτ-methylhistamine.
• This enzyme plays a critical role in histamine metabolism and degradation, regulating histamine levels in various tissues including the airway, thereby modulating the airway response to histamine.
• HNMT localizes to the cytoplasm, as indicated by subcellular location data and keywords.
• The HNMT gene undergoes alternative splicing, producing multiple isoforms such as HNMT-S1 and HNMT-S2, which may have distinct functional properties.
• Protein domain analysis classifies HNMT as a methyltransferase, requiring S-adenosyl-L-methionine (SAM) as a methyl donor for its catalytic activity.
• Mutations in HNMT have been associated with disease variants and intellectual disability, highlighting its clinical relevance.
• Keywords from UniProt include 3D-structure, alternative splicing, cytoplasm, disease variant, intellectual disability, methyltransferase, S-adenosyl-L-methionine, and transferase, indicating its structural characterization and functional classification.

Experimental Guidance and Technical Tips

• For Western blotting (WB), consider testing dilution factors in the range of 1:500–1:2000 using lysates from human or mouse tissues known to express HNMT, such as lung or kidney.
• As a polyclonal antibody generated against full-length recombinant HNMT, this reagent may detect multiple isoforms (e.g., HNMT-S1, HNMT-S2) due to epitope coverage across the protein sequence.
• In immunoprecipitation (IP) experiments, validate the antibody’s ability to pull down native HNMT under non-denaturing conditions; inclusion of appropriate controls is recommended.
• For ELISA, the purified recombinant protein can be used as a standard to quantify antibody reactivity; consider optimizing coating and blocking conditions.
• Cross-reactivity with mouse HNMT suggests conserved epitopes, but verification in the relevant mouse model system is advised before critical comparative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw-material sourcing, offering a wide range of antibodies, antigens, and ancillary reagents essential for diagnostic development and research. This HNMT Rabbit pAb supports studies in histamine metabolism, allergy, and respiratory diseases, aligning with CamelBio's commitment to providing validated tools from concept to clinic. Explore our portfolio for integrated solutions including matched antibody pairs, recombinant proteins, and customized procurement services to streamline your assay development.