Anti-HNMT Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P50135

Core Product Specifications and Parameters

Biological Background: HNMT Function and Localization

• HNMT (Histamine N-methyltransferase) is a cytoplasmic enzyme that catalyzes the N-methylation of histamine, utilizing S-adenosyl-L-methionine as a methyl donor, thereby inactivating histamine.
• It plays a critical role in histamine degradation and in modulating airway responsiveness to histamine.
• Alternative splicing generates multiple isoforms, including HNMT-S1 and HNMT-S2.
• The protein is localized to the cytoplasm.
• It belongs to the methyltransferase family and is classified under the transferase group of enzymes.
• Mutations in HNMT have been associated with intellectual disability.
• Proteomic identification confirms its expression in human tissues.

Experimental Guidance and Technical Tips

• Based on the immunogen design, this polyclonal antibody may recognize multiple HNMT isoforms; validate in your sample system.
• For Western blot (WB), a band around 33 kDa is anticipated; optimize loading and transfer conditions.
• For IF/ICC, cytoplasmic staining is expected; include appropriate controls.
• In ELISA and IP applications, consider pairing with other HNMT antibodies for sandwich formats.
• Always titrate the antibody to determine optimal working dilutions for your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-HNMT antibody for histamine metabolism and airway response studies. Our one-stop sourcing model supports your workflow from concept to clinic, offering bulk ancillary reagents and assistance in procuring rare-target raw materials.