Anti-HNF4G Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q14541

Core Product Specifications and Parameters

Biological Background: HNF4G Function and Localization

• HNF4G (Hepatocyte nuclear factor 4-gamma) is a transcription factor belonging to the nuclear receptor subfamily 2 group A, encoded by the NR2A2 gene.
• It exhibits lower transcriptional activation potential compared to the related HNF4-alpha isoform, suggesting distinct regulatory roles.
• The protein features zinc-finger domains, conferring DNA-binding and metal-binding capabilities, and undergoes phosphorylation, as indicated by proteomic studies.
• HNF4G localizes exclusively to the nucleus, consistent with its function as a transcriptional regulator.
• Tissue expression is highly specific: abundant in pancreas, kidney, small intestine, and testis; weakly present in colon; and absent in liver, skeletal muscle, lung, placenta, brain, heart, peripheral blood, ovary, prostate, thymus, and spleen.
• As a nuclear receptor, HNF4G is involved in the regulation of genes critical for metabolic and developmental processes.
• Alternative splicing generates multiple isoforms, potentially modulating its activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 319–408 near the C-terminus of human HNF4G; the antibody may recognize epitopes within this region and should be validated in the relevant sample system.
• For Western blotting, the predicted molecular weight is approximately 46 kDa (45.9 kDa based on UniProt sequence); consider loading adequate protein amounts, especially in tissues with low expression.
• In IHC-P, optimize antigen retrieval and antibody dilution; positive control tissues such as pancreas or kidney are recommended based on expression data.
• ELISA applications may require titration to determine optimal coating antigen concentration and antibody dilution for quantitative detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated monoclonal and polyclonal antibodies for transcription factors like HNF4G. Beyond this product, we offer antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, streamlining your supply chain from discovery to clinical application. Our integrated platform ensures reliable access to high-quality reagents for transcriptional regulation studies and beyond.