Anti-HNF4G Polyclonal Antibody for WB, IF/ICC, ELISA - Q14541

Core Product Specifications and Parameters

Biological Background: HNF4G Function and Localization

• HNF4G, also known as NR2A2 or nuclear receptor subfamily 2 group A member 2, is a member of the hepatocyte nuclear factor 4 family of transcription factors.
• It functions as a transcription factor, though with lower transcriptional activation potential compared to HNF4-alpha (HNF4A).
• HNF4G localizes predominantly to the nucleus.
• Tissue expression is highest in pancreas, kidney, small intestine and testis, with weak expression in colon; it is absent in liver, skeletal muscle, lung, placenta, brain, heart, peripheral blood, ovary, prostate, thymus, and spleen.
• The protein contains zinc-finger DNA-binding domains and is classified as a metal-binding phosphoprotein, indicative of regulatory post-translational modifications.
• Alternative splicing generates multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment spanning amino acids 366-445; consider using a positive control lysate from tissues such as pancreas or kidney (where HNF4G is highly expressed) for Western blot.
• For ICC/IF, validate nuclear staining in HNF4G-expressing cell lines or tissue sections, using appropriate fixation and permeabilization protocols.
• In ELISA applications, use recombinant HNF4G protein as a standard to establish assay sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and researchers to supply premium IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This HNF4G polyclonal antibody supports studies in nuclear receptor biology and transcription factor research. For rare target sourcing or custom development projects, CamelBio offers a streamlined bridge from concept to clinic.