Anti-HNF4A Polyclonal Antibody for WB and ELISA - P41235

Core Product Specifications and Parameters

Biological Background: Hepatocyte Nuclear Factor 4-alpha Function and Localization

• HNF4A, also known as nuclear receptor subfamily 2 group A member 1 (NR2A1) and transcription factor 14 (TCF14), is a member of the nuclear receptor family that functions as a ligand-activated transcription factor.
• In the liver, HNF4A controls the expression of hepatic genes during endodermal-to-hepatic progenitor cell differentiation by facilitating RNA polymerase II recruitment to target promoters. Related references: PMID:30597922
• It represses the CLOCK-BMAL1 transcriptional complex and is essential for circadian rhythm maintenance and period regulation in liver and colon cells. Related references: PMID:30530698
• HNF4A localizes exclusively to the nucleus, where it binds DNA at specific response elements.
• Post-translational modifications, including acetylation, phosphorylation, and ubiquitination-like conjugation, modulate its transcriptional activity and protein stability.
• Mutations in HNF4A are associated with maturity-onset diabetes of the young type 1 (MODY1) and other metabolic disorders, highlighting its role in glucose homeostasis.
• Alternative promoter usage and splicing generate multiple isoforms (e.g., HNF4a7, HNF4a8, HNF4a9), which may have tissue-specific functions.
• The protein contains conserved zinc-finger domains for DNA binding and a ligand-binding domain characteristic of nuclear receptors.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to a sequence within amino acids 226–325 of human HNF4A. Consider verifying isoform reactivity if targeting specific splice variants.
• For Western blotting, the predicted protein weight is 53 kDa. Using nuclear extracts from human liver or colon tissue/cells may improve signal.
• Confirm specificity via knockdown or overexpression experiments, and include appropriate positive and negative controls.
• In ELISA, the immunogen peptide can serve as a positive control; test against full-length recombinant protein to validate binding.
• Polyclonal antibodies can exhibit lot-to-lot variation; re-standardize your protocol when using a new batch.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with reliable access to high-quality IVD raw materials, including validated antibodies for transcription factors like HNF4A. From optimized polyclonal and monoclonal antibodies to bulk ancillary reagents, our one-stop platform supports your assay development from concept to commercialization. Reach out to explore customized sourcing solutions for rare targets in liver metabolism and genetic disease research.