Anti-HNF3β/FOXA2 Rabbit Monoclonal Antibody for WB, IF/ICC, IP, ELISA - Q9Y261

Core Product Specifications and Parameters

Biological Background: HNF3β/FOXA2 Function and Localization

• HNF3β/FOXA2, also known as Forkhead box protein A2 or TCF-3B, is a transcription factor encoded by the FOXA2 gene in humans.
• Acts as a pioneer transcription factor by binding nucleosomal core histones, displacing linker histones, and opening compacted chromatin at target enhancers/promoters to facilitate gene regulation (By similarity).
• Essential for embryonic development, including notochord formation and development of endoderm-derived organs such as the liver, pancreas, and lungs; functions partially redundantly with FOXA1.
• Regulates expression of liver-specific genes like AFP, albumin, tyrosine aminotransferase, and PEPCK by interacting with their cis-regulatory elements.
• Involved in glucose homeostasis by controlling pancreatic β-cell gene expression and modulates fat metabolism; binds fibrinogen beta promoter and mediates IL6-induced transcriptional activation.
• Localizes predominantly to the nucleus but can also be found in the cytoplasm.
• Post-translationally modified by phosphorylation; its DNA-binding domain recognizes the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3'.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide covering the N-terminal region (aa 1-100) of human HNF3β/FOXA2; sequence conservation in other species or isoforms should be examined via alignment if cross-reactivity is desired.
• This rabbit monoclonal antibody is indicated for WB, IF/ICC, IP, and ELISA according to the supplier; each assay requires individual optimization.
• For WB, the predicted molecular weight is 48kDa; however, post-translational modifications may cause minor shifts. Validate in the relevant sample system and consider appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material procurement, bridging research and clinical manufacturing. This anti-HNF3β/FOXA2 rabbit monoclonal antibody exemplifies our portfolio of transcription factor targets applied in metabolic, developmental, and oncology research. From validated antibody pairs and bulk ancillary reagents to custom rare target sourcing, we provide the comprehensive supply chain support needed to advance your diagnostic development.