Anti-HNF1α Rabbit Polyclonal Antibody for WB, ELISA - P20823

Core Product Specifications and Parameters

Biological Background: HNF1α Function and Localization

• Hepatocyte nuclear factor 1-alpha (HNF1α) is a transcription factor encoded by the HNF1A gene; alternative splicing produces multiple isoforms (UniProt KB keywords: Alternative splicing).
• Functions as a transcriptional activator that regulates tissue-specific gene expression, primarily in pancreatic islet cells and liver (By similarity). It binds to the inverted palindrome 5'-GTTAATNATTAAC-3' to control target gene transcription (PubMed:10966642, PubMed:12453420). Related references: PMID:10966642 PMID:12453420
• Activates the transcription of cytochrome P450 enzymes such as CYP1A2, CYP2E1, and CYP3A11, linking it to drug metabolism pathways (By similarity).
• Plays a crucial role in hepatitis B virus (HBV) gene transcription and DNA replication by synergizing with NR5A2 to enhance the viral enhancer II (ENII) activity (Microbial infection).
• Subcellular localization is exclusively nuclear, consistent with its role as a DNA-binding transcription factor (UniProt: Nucleus).
• Expressed predominantly in the liver, where it controls a network of hepatocyte-specific genes (UniProt tissue specificity: Liver).
• Post-translational modifications include phosphorylation and ubiquitin conjugation (Keywords: Phosphoprotein, Ubl conjugation), and the protein is known to contain a homeobox domain (Keywords: Homeobox, DNA-binding).
• Clinically, mutations in HNF1A are associated with maturity-onset diabetes of the young type 3 (MODY3) and diabetes mellitus (Keywords: Diabetes mellitus, Disease variant).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 30–130 of human HNF1α, which encompasses portions of the N-terminal domain; consider potential cross-reactivity with other homeobox-containing proteins and validate specificity using knockout or siRNA-based controls.
• For Western blotting, the predicted molecular weight is approximately 67 kDa; use positive control lysates from liver or pancreatic islet cell lines (e.g., HepG2 or MIN6) to confirm the band. Migrated bands may shift due to phosphorylation or other modifications; phosphatase treatment can help verify modification status.
• In ELISA applications, optimize coating antigen concentrations and detection conditions; include recombinant HNF1α protein or peptide competition assays to assess antibody specificity.
• The antibody is reported to cross-react with mouse and rat HNF1α; if using in other species, perform additional validation experiments to confirm reactivity.
• Given the involvement of HNF1α in hepatitis B virus biology, this antibody may be useful in studying HBV-host interactions; however, appropriate viral models and controls are necessary.

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CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering diagnostic manufacturers, laboratories, and research institutes streamlined access to high-quality reagents like this HNF1α polyclonal antibody, along with technical services and consulting from concept to clinic. Whether you are developing assays for liver disease biomarkers, diabetes diagnostics, or transcription factor research, our supply capabilities encompass validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare targets. Partner with CamelBio to simplify your supply chain and secure reliable materials at every stage of IVD development.