Anti-HNF-4-alpha Monoclonal Antibody for WB, IHC-P, ELISA, and ChIP - P49698

Core Product Specifications and Parameters

Biological Background: HNF-4-alpha Function and Localization

• HNF-4-alpha (Hepatocyte Nuclear Factor 4-alpha) is a nuclear receptor transcription factor that controls the expression of hepatic genes during endodermal-to-hepatic progenitor cell transition, facilitating RNA polymerase II recruitment to target gene promoters (by similarity).
• It activates transcription of CYP2C38, a cytochrome P450 enzyme involved in drug metabolism.
  Related references:
  PMID:30555544
• HNF-4-alpha represses the CLOCK-BMAL1 transcriptional complex, playing an essential role in circadian rhythm maintenance and period regulation in the liver and colon.
  Related references:
  PMID:30530698
• The protein is primarily localized in the nucleus, consistent with its function as a transcription factor.
• It is expressed in a circadian manner in the liver, pancreas, and colon, reflecting its tissue-specific regulatory roles.
• HNF-4-alpha undergoes post-translational modifications including acetylation and phosphorylation, and features zinc-finger domains characteristic of nuclear receptors.

Experimental Guidance and Technical Tips

• The antibody was raised against amino acids 1-200 of mouse HNF-4-alpha, an N-terminal region that encompasses part of the DNA-binding domain. For ChIP assays, consider using this antibody to enrich genomic DNA fragments bound by HNF-4-alpha.
• In Western blot (WB), HNF-4-alpha is expected to migrate at approximately 53 kDa; validate detection in nuclear extracts from liver or colon tissue, where it is endogenously expressed.
• For immunohistochemistry (IHC-P), provide robust tissue fixation and antigen retrieval steps; as a nuclear protein, anticipate nuclear staining.
• The antibody has demonstrated cross-reactivity with human, mouse, and rat, making it suitable for comparative studies across these species. However, always verify specificity in your specific experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw materials, offering a streamlined path from concept to clinic. This anti-HNF-4-alpha monoclonal antibody supports research in liver biology, metabolic disorders, and circadian rhythm. We provide validated antibody pairs, bulk monoclonal antibodies, ancillary reagents, and can source rare-target raw materials to meet your diagnostic development needs.