Anti-HN1 Rabbit Monoclonal Antibody for WB, ELISA - Q9UK76

Core Product Specifications and Parameters

Biological Background: HN1 (JPT1) Function and Localization

• HN1 (JPT1, also known as Jupiter microtubule associated homolog 1, ARM2, or hematological and neurological expressed 1 protein) is a ubiquitously expressed protein with a predicted molecular weight of ~16 kDa.
• It negatively modulates AKT-mediated GSK3B signaling, impacting downstream pathways. Related references: PMID:21323578 PMID:22155408
• HN1 promotes CTNNB1 (beta-catenin) phosphorylation at Ser-33 and its subsequent degradation by suppressing inhibitory phosphorylation of GSK3B, thereby weakening the APC:CTNNB1:GSK3B complex and CDH1/E-cadherin interactions in adherent junctions. Related references: PMID:25169422
• The protein contributes to the regulation of cell cycle progression and cell adhesion. Related references: PMID:25169422 PMID:25450365
• HN1 exerts an inhibitory effect on the androgen receptor (AR) signaling pathway by inducing receptor proteasomal degradation. Related references: PMID:22155408
• It localizes to both the nucleus and cytoplasm, consistent with its roles in transcriptional and cytoplasmic signaling events.
• Tissue expression is broad, with notable levels in testis, skeletal muscle, thymus, prostate, colon, peripheral blood cells, brain, and placenta.
• The protein undergoes acetylation and phosphorylation; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50-154 of human HN1, a region likely involved in protein-protein interactions; this design may favor detection of native conformations.
• For Western blot, consider testing whole-cell lysates from human cell lines such as LNCaP or HeLa, where HN1 is endogenously expressed; optimize antibody dilution and detection conditions empirically.
• In ELISA, the recombinant immunogen can be used as a positive control; ensure appropriate plate coating and blocking steps.
• Validate specificity through siRNA knockdown or knockout cell lines if available, to confirm band identity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material solutions, including this HN1 (JPT1) monoclonal antibody, tailored for oncology and androgen signaling research. We offer validated antibody pairs, bulk ancillary reagents, and can assist in sourcing rare targets to support your diagnostic assay development from concept to clinic.