Anti-HMOX2 Rabbit Polyclonal Antibody for WB, ELISA - P30519

Core Product Specifications and Parameters

Biological Background: HMOX2 Function and Localization

• HMOX2 (also known as HO-2) is a heme oxygenase enzyme that catalyzes the rate-limiting step in heme catabolism.
• It cleaves heme at the alpha-methene bridge to produce equimolar amounts of biliverdin IXalpha, carbon monoxide (CO), and free ferrous iron.
• HMOX2 is an integral membrane protein located on the cytoplasmic face of the endoplasmic reticulum and microsomal membranes.
• The enzyme contains a conserved heme-binding pocket and requires heme as a cofactor for its oxidoreductase activity.
• Post-translational modifications include acetylation, phosphorylation, and S-nitrosylation, which may regulate its activity or stability.
• Alternative splicing generates multiple transcript variants encoding different isoforms of the protein.
• HMOX2 plays a critical role in cellular iron homeostasis and in the generation of the gaseous signaling molecule CO.
• It belongs to the heme oxygenase family and is a reference proteome component with published 3D structural data.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-270 of human HMOX2, which includes the entire heme oxygenase domain; therefore, the antibody may recognize multiple isoforms and species orthologs.
• For Western blot, a band corresponding to approximately 36 kDa is expected. Additional bands may be observed due to post-translational modifications or alternative splicing variants.
• Cross-reactivity with mouse and rat HMOX2 has been reported, but sequence alignment should be checked for other species before use.
• For ELISA applications, consider using this polyclonal antibody as a capture or detection reagent after appropriate titration and optimization.
• As a rabbit polyclonal antibody, it may offer enhanced sensitivity for detecting low-abundance endogenous HMOX2; however, lot-to-lot consistency should be verified for quantitative studies.
• It is advisable to include appropriate positive and negative controls, such as heme oxygenase 2 overexpressing lysates or tissues known to express HMOX2 (e.g., testes, brain).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers and research labs with comprehensive access to high-quality raw materials for heme metabolism research, including validated antibodies like this HMOX2 polyclonal antibody. Our extensive portfolio covers antibody pairs, recombinant proteins, and ancillary reagents, enabling efficient assay development from concept to clinic. Whether you need bulk quantities or custom sourcing for rare targets, CamelBio is your strategic partner in IVD raw material procurement.