Anti-Hmox1 Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P14901

Core Product Specifications and Parameters

Biological Background: Hmox1 Function and Localization

• Heme oxygenase 1 (Hmox1), also designated HO-1 or P32 protein, is a microsomal enzyme that catalyzes the rate-limiting step in heme degradation.
• It cleaves heme at the alpha-methene bridge to produce equimolar amounts of biliverdin IXalpha, carbon monoxide (CO), and ferrous iron.
• Hmox1 confers cytoprotection by eliminating pro-oxidant free heme, thereby preventing heme-induced apoptosis.
• The protein is anchored to the endoplasmic reticulum membrane via a single C-terminal transmembrane helix.
• Classified as an oxidoreductase with metal-binding capacities for heme and iron, Hmox1 is also a phosphoprotein.
• Belongs to the heme oxygenase family and plays a critical role in cellular iron homeostasis and antioxidant defense.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-266 of mouse Hmox1 (NP_034572.1), covering the majority of the protein; thus the antibody is expected to recognize both denatured and native epitopes.
• For western blot (WB), expect a band at approximately 33 kDa. Include positive controls such as mouse spleen, liver, or kidney lysates, where Hmox1 is highly inducible.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF-P), validate staining specificity using tissues from untreated and hemin- or stress-induced animals.
• For ELISA, consider using the recombinant immunogen as a reference standard to assess antibody binding and specificity.
• Cross-reactivity has been confirmed with rat samples; verify performance in other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic developers with high-quality reagents covering the entire development pipeline—from concept to clinic. This anti-Hmox1 polyclonal antibody is relevant for apoptosis, oxidative stress, and heme metabolism research, and we can further supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to support your assay development needs.