Anti-HMGN1 Polyclonal Antibody for WB, IF/ICC, ELISA - P05114

Core Product Specifications and Parameters

Biological Background: HMGN1 Function and Localization

• HMGN1, also known as High mobility group nucleosome-binding domain-containing protein 1 or HMG14, is a non-histone chromosomal protein that binds specifically to nucleosomal DNA.
• It interacts with the inner side of the nucleosomal DNA, altering DNA-histone interactions and modulating chromatin structure.
• HMGN1 is implicated in maintaining transcriptionally active genes in a unique chromatin conformation, facilitating gene expression.
• The protein inhibits phosphorylation of nucleosomal histones H3 and H2A by kinases RPS6KA5/MSK1 and RPS6KA3/RSK2, suggesting a role in chromatin signaling (by similarity).
• Subcellular localization includes the nucleus and cytoplasm, indicating dynamic shuttling or extranuclear functions.
• Post‑translational modifications of HMGN1 include acetylation, ADP‑ribosylation, and phosphorylation; it is classified as a phosphoprotein.
• Additional annotations highlight its DNA‑binding property, involvement in RNA editing, and status as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N‑terminal region (amino acids 1–100) of human HMGN1; consider this when designing epitope‑specific detection strategies.
• Applications validated by the supplier include WB, IF/ICC, and ELISA; however, optimal working dilutions must be determined experimentally in the relevant sample system.
• As a polyclonal antibody, batch‑to‑batch consistency should be confirmed for critical assays; consider internal reference standards.
• Reported cross‑reactivity with mouse HMGN1 makes this antibody suitable for studies in mouse models, but cross‑reactivity in other species should be independently verified.
• HMGN1 is a small (~11 kDa) chromatin protein; extend blocking and washing steps in Western blotting to improve signal for low‑molecular‑weight targets.
• For immunofluorescence, permeabilization may be critical to detect nuclear localization; optimize fixation and permeabilization protocols accordingly.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio enables diagnostic manufacturers, labs, and research institutes to source IVD raw materials effortlessly, from concept to clinic. This anti‑HMGN1 polyclonal antibody supports chromatin and gene expression research, and CamelBio can supply additional validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and assistance in rare‑target sourcing. Reach out to streamline your complete IVD raw‑material procurement with one reliable partner.