Anti-HMGCS2 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P54868

Core Product Specifications and Parameters

Biological Background: HMGCS2 Function and Localization

• HMGCS2 encodes mitochondrial hydroxymethylglutaryl-CoA synthase, which catalyzes the first irreversible step of ketogenesis by condensing acetyl-CoA with acetoacetyl-CoA to form HMG-CoA.
• The enzyme is a transferase involved in lipid metabolism, specifically in cholesterol biosynthesis and steroid/sterol metabolic pathways.
• It belongs to the HMGCS gene family, functioning as a mitochondrial isoform distinct from the cytosolic HMGCS1.
• Subcellular localization: strictly mitochondrial, where it carries out its metabolic function; a transit peptide directs its import.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity; the protein is also alternatively spliced.
• Tissue expression: primarily liver, with levels 200-fold higher than other tissues; low expression in colon, kidney, testis, and pancreas; some studies report highest expression in heart and skeletal muscle, though detection methods may vary.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 269-508 of human HMGCS2 (NP_005509.1). This region is within the catalytic domain, potentially ensuring recognition of native and recombinant protein.
• Recommended for Western blot (WB), immunofluorescence on paraffin-embedded sections (IF-P), immunohistochemistry on paraffin-embedded sections (IHC-P), and ELISA. Validate antibody concentration and protocol in your specific sample system.
• Cross-reactivity confirmed with human, mouse, and rat; consider sequence alignment for additional species if needed.
• Mitochondrial localization requires appropriate sample preparation (e.g., subcellular fractionation) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated IVD raw material solutions, from concept to clinic. As a sourcing partner, we offer this anti-HMGCS2 antibody alongside validated antibody pairs, bulk ancillary reagents, and customized supply of rare targets to support ketogenesis and metabolic disorder research. Let us streamline your procurement with reliable, scalable materials.